Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Chicken Cell (neural fold stage)
Specification
neural fold stage
Permeabilization
Yes - 0.3% tritonX-100 in PBS
Fixative
10% trichloroacetic acid
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% natural goat serum in 0.3%Triton
Dilution
1/25
Secondary antibody
Name
Non-Abcam antibody was used: alexa fluor goat anti-rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Did not work on embryos fixed with 4% PFA for 1 hour
Used on neural fold stage chick embryos fixed with 10% trichloroacetic acid in 1xPBS for 1 hour at 4C. Embryos were rinsed in 5% sucrose and 30% sucrose and embedded in OCT. Embryos were cryosectioned at 16um. Cldn-3 was expressed in the non-neural ectoderm and in the endoderm, but not in the neural ectoderm. This expression pattern matches the RNA expression pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Jean-Francois Boisclair Lachance
Verified customer
提交于 Jun 25 2014