Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH 9.0
Permeabilization
Yes - PBS-Triton 0,1% 10min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/300
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-Triton 0,1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: ALEXAFluor™ 488 GOAT ANTI RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Slides were rehydrated and then boiled for antigen retrieval in TE pH 9 buffer for 15min in standard microwave oven at 650W. After 30min blocking in PBS-Triton 0,1% / BSA 5% slides were incubated O/N with primary antibody at 1/300 dilution at 4°C. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/200 dilution for 1h. Nuclei were stained with Hoechst. Slides were mounted with VECTASHIELD mounting solution.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Daniel Alpern
Verified customer
提交于 Aug 06 2012