Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (4-15% Tris-glycine)
Sample
Pig Recombinant protein (Citrate Synthase from porcine heart (Sigma))
Specification
Citrate Synthase from porcine heart (Sigma)
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: OBB (TBS) + 0.2% Tween20
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/10000
Detection
Detection method
LI-COR infrared imaging
Bands
Specific: 50 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Rachel Loh
Verified customer
提交于 Jan 15 2015