Anti-Chromogranin A抗体(ab45179)
Key features and details
- Rabbit polyclonal to Chromogranin A
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Chromogranin A抗体
参阅全部 Chromogranin A 一抗 -
描述
兔多克隆抗体to Chromogranin A -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Dog, Pig, Monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: PC-12 and SH-SY5Y cell lysates. IHC-P: Human pancreatic tissue and adenocarcinoma of pancreas tissue. ICC/IF: Panc-1 and SHSY5Y cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45179于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (8) |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF |
Use a concentration of 5 µg/ml.
For unpurified use at 1/50 - 1/100. |
|
WB |
1/2000. Detects a band of approximately 86 kDa.
For unpurified use at 1/10000. |
说明 |
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IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 5 µg/ml. For unpurified use at 1/50 - 1/100. |
WB
1/2000. Detects a band of approximately 86 kDa. For unpurified use at 1/10000. |
靶标
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功能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
序列相似性
Belongs to the chromogranin/secretogranin protein family. -
翻译后修饰
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
细胞定位
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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数据库链接
- Entrez Gene: 1113 Human
- Entrez Gene: 12652 Mouse
- Entrez Gene: 397540 Pig
- Entrez Gene: 24258 Rat
- Omim: 118910 Human
- SwissProt: P10645 Human
- SwissProt: P26339 Mouse
- SwissProt: P04404 Pig
see all -
形式
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
别名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
图片
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Unpurified ab45179 staining Chromogranin A in pig small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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Anti-Chromogranin A antibody (ab45179) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 86 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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ab45179 staining Chromogranin A in SHSY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab45179 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of pancreas tissue labelling Chromogranin A with purified ab45179 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Chromogranin A antibody (ab45179) at 1/1000 dilution (purified) + PC-12 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 86 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue labelling Chromogranin A with purified ab45179 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Chromogranin A antibody (ab45179) at 1/10000 dilution (unpurified) + PC-12 cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Observed band size: 86 kDa why is the actual band size different from the predicted? -
Chromogranin A staining on monkey pancreas sections. The sections were subjected to heat mediated epitope retrieval using citric acid (pH 6) and blocked using 1% BSA for 10 mins at 21°C. Unpurifeid ab45179 was used at a dilution of 1/750 using TBS/BSA/Azide for 2 hours at 21°C. (Red arrowheads indicate extra Islet positive cells).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab45179 被引用在 26 文献中.
- Wang M et al. The developmental changes in intestinal epithelial cell proliferation, differentiation, and shedding in weaning piglets. Anim Nutr 9:214-222 (2022). PubMed: 35600553
- Perdigoto AL et al. Immune cells and their inflammatory mediators modify β cells and cause checkpoint inhibitor-induced diabetes. JCI Insight 7:N/A (2022). PubMed: 35925682
- Chen X et al. Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer. Cell Death Dis 13:927 (2022). PubMed: 36335093
- Engevik MA et al. Human-Derived Bifidobacterium dentium Modulates the Mammalian Serotonergic System and Gut-Brain Axis. Cell Mol Gastroenterol Hepatol 11:221-248 (2021). PubMed: 32795610
- Kasashima H et al. Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population. Dev Cell 56:95-110.e10 (2021). PubMed: 33207226