Anti-Chromogranin A抗体(ab15160)
Key features and details
- Rabbit polyclonal to Chromogranin A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Chromogranin A抗体
参阅全部 Chromogranin A 一抗 -
描述
兔多克隆抗体to Chromogranin A -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Human
预测可用于: Mouse, Monkey -
免疫原
Recombinant fragment within Human Chromogranin A aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P10645 -
阳性对照
- IHC-P: FFPE human normal pancreas tissue
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15160于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (22) |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
序列相似性
Belongs to the chromogranin/secretogranin protein family. -
翻译后修饰
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
细胞定位
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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数据库链接
- Entrez Gene: 1113 Human
- Entrez Gene: 12652 Mouse
- Omim: 118910 Human
- SwissProt: P10645 Human
- SwissProt: P26339 Mouse
- Unigene: 150793 Human
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形式
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
别名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
图片
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IHC image of Chromogranin A staining in a section of formalin-fixed paraffin-embedded normal human pancreas* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15160, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
数据表及文件
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SDS download
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Datasheet download
文献 (218)
ab15160 被引用在 218 文献中.
- Kim H et al. Baiap3 regulates depressive behaviors in mice via attenuating dense core vesicle trafficking in subsets of prefrontal cortex neurons. Neurobiol Stress 16:100423 (2022). PubMed: 35028340
- Ishii J et al. Endocrine secretory granule production is caused by a lack of REST and intragranular secretory content and accelerated by PROX1. J Mol Histol 53:437-448 (2022). PubMed: 35094211
- Wang J et al. Nfatc1+ colonic stem cells contribute to regeneration upon colitis. J Gastroenterol Hepatol 37:734-740 (2022). PubMed: 35102609
- Saari J et al. Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35163236
- Walter RJ et al. Wnt signaling is boosted during intestinal regeneration by a CD44-positive feedback loop. Cell Death Dis 13:168 (2022). PubMed: 35190527