Chromatin Extraction试剂盒(ab117152)
Key features and details
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
概述
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产品名称
Chromatin Extraction试剂盒
参阅全部 Chromatin Extraction 试剂盒 -
样品类型
Tissue, Adherent cells, Suspension cells -
检测时间
1h 00m -
产品概述
Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 100 tests 1000X Protease Inhibitor Cocktail 1 x 110µl 10X Lysis Buffer 1 x 11ml Chromatin Buffer 1 x 11ml Extraction Buffer 1 x 11ml -
研究领域
图片
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ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
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Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A. Chromatin yield was 4 µg chromatin/106 cells. Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments
Image courtesy of anonymous Abreview
数据表及文件
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Datasheet download
文献 (38)
ab117152 被引用在 38 文献中.
- Prieto-Garcia C et al. Inhibition of USP28 overcomes Cisplatin-resistance of squamous tumors by suppression of the Fanconi anemia pathway. Cell Death Differ 29:568-584 (2022). PubMed: 34611298
- Jin X et al. Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma. Oncogene 41:538-549 (2022). PubMed: 34785774
- Malovitski K et al. Loss-of-function variants in KLF4 underlie autosomal dominant palmoplantar keratoderma. Genet Med 24:1085-1095 (2022). PubMed: 35168889
- Li Y et al. WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma. Cell Death Discov 8:107 (2022). PubMed: 35264565
- Liu W et al. The RNF26/CBX7 axis modulates the TNF pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC. Int J Biol Sci 18:2132-2145 (2022). PubMed: 35342353