Application
Western blot
Sample
Rhesus monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
100 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/1% FBS
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 72 kDa Non-specific: 30 kDa
Additional data
Additional Notes
ab6168 recognized choline acetyltransferase in macaque retina lysates. Two bands of 72 kDa (expected) and 30 kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Monica Sauter
Verified customer
提交于 Jan 11 2016