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RabMAb

Anti-Chk2 (phospho T68)抗体[Y171] (ab32148)

概述

  • 产品名称
    Anti-Chk2 (phospho T68)抗体[Y171]
    参阅全部 Chk2 一抗
  • 描述
    兔单克隆抗体[Y171] to Chk2 (phospho T68)
  • 特异性
    The antibody will detect Chk2 phosphorylation on Threonine 68. This antibody may also detect all splice isoforms (2-12) based on sequence homology.
  • 经测试应用
    适用于: IP, WB, Dot blotmore details
    不适用于: Flow Cyt,ICC or IHC
  • 种属反应性
    与反应: Human
  • 免疫原

    corresponding to Human Chk2.

  • 阳性对照
    • Untreated HEK-293 cell lysates, cell lysates from HEK-293 cells treated with UV and/or phosphatase.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/20 - 1/50.
WB 1/1000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 61 kDa).
Dot blot 1/500.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC.
  • 靶标

    • 功能
      Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
    • 组织特异性
      High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
    • 疾病相关
      Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
      Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
      Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
    • 序列相似性
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
      Contains 1 FHA domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Phosphorylated by PLK4.
    • 细胞定位
      Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDS 1 antibody
      • Cds1 antibody
      • Cds1 homolog antibody
      • Checkpoint kinase 2 antibody
      • Checkpoint like protein CHK2 antibody
      • CHEK 2 antibody
      • Chek2 antibody
      • Chk 2 antibody
      • CHK2 checkpoint homolog (S. pombe) antibody
      • CHK2 checkpoint homolog antibody
      • CHK2_HUMAN antibody
      • hCds1 antibody
      • HuCds 1 antibody
      • LFS 2 antibody
      • LFS2 antibody
      • PP1425 antibody
      • RAD 53 antibody
      • RAD53 antibody
      • Rad53 homolog antibody
      • Serine/threonine protein kinase Chk2 antibody
      • Serine/threonine-protein kinase Chk2 antibody
      see all

    Anti-Chk2 (phospho T68) antibody [Y171] 图像

    • All lanes : Anti-Chk2 (phospho T68) antibody [Y171] (ab32148) at 1/5000 dilution

      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : Whole cell lysate from HEK-293 (Human epithelial cell line from embryonic kidney) cells, treated with UV at 60J/m2 for 2 hours.
      Lane 3 : Whole cell lysate from HEK-293 (Human epithelial cell line from embryonic kidney) cells, treated with UV at 60J/m2 for 2 hours. Membrane incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 61 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds

      Blocking/dilution buffer: 5% NFDM/TBST

    • ab32148 at 1/20 dilution immunoprecipitating Chk2 (phospho T68) in HEK-293 (human embryonic kidney) whole cell lysate observed at 70kDa (lanes 1 and 2).

      Lane 1 (input): HEK-293 cells treated with UV at 60J/m2 for 2 hours. Whole cell lysate 10μg

      Lane 2 (+): ab32148 + HEK-293 treated with UV at 60J/m2 for 2 hours. Whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32148 in HEK- treated with UV at 60J/m2 for 2 hours. Whole cell lysate

      For western blotting, ab32148 at 1/500 dilution was used followed by ab131366 VeriBlot for IP (HRP) at 1/1000 as the secondary antibody.

      Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-Chk2 (phospho T68) antibody [Y171] (ab32148) at 1/1000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : 293 cell lysate treated with 2uM Doxorubicin
      Lane 3 : 293 cell lysate treated with 10uM Doxorubicin


      Predicted band size : 61 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    • Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Phospho-Chkr2 (pT68) RabMAb (ab32148) dilution 1:500
    • All lanes : Anti-Chk2 (phospho T68) antibody [Y171] (ab32148) at 1/1000 dilution

      Lane 1 : HCT116 treated with DMSO (control) whole cell lysate
      Lane 2 : HCT116 treated with 10 µM etoposide for 24 hours whole cell lysate

      Lysates/proteins at 60 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 61 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 20 seconds

      Non-specific band : 35, 30 kDa

      Blocking/Diluting buffer : 5% Milk

       

      See Abreview

    Anti-Chk2 (phospho T68) antibody [Y171] (ab32148)参考文献

    This product has been referenced in:
    • Song H  et al. ATG16L1 phosphorylation is oppositely regulated by CSNK2/casein kinase 2 and PPP1/protein phosphatase 1 which determines the fate of cardiomyocytes during hypoxia/reoxygenation. Autophagy 11:1308-25 (2015). Read more (PubMed: 26083323) »
    • Li WF  et al. WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS One 9:e94751 (2014). Human . Read more (PubMed: 24728101) »

    See all 4 Publications for this product

    Product Wall

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (lymphocytes)
    Specification
    lymphocytes
    Treatment
    UV treated
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 07 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 colon cancer cell line)
    Loading amount
    60 µg
    Specification
    HCT116 colon cancer cell line
    Treatment
    DMSO control and 10 µM etoposide for 24 hrs
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Christian Marx

    Verified customer

    提交于 Oct 29 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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