Application
Western blot
Sample
Human Cell lysate - nuclear (Vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
100 uM H2O2 2 hours
Specification
Vascular smooth muscle cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye 800CW
Detection
Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 61 kDa
Positive control
Second lane is treated with H2O2 to induce DNA damage.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 31 2017