Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell lysate)
Loading amount
22 µg
Specification
HeLa cell lysate
Treatment
+/- 10 Gy IR
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/10000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% BSA in TBS-Tween20
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG, HRP-linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
BioRad Clarity ECL
Exposure
5 second(s)
Bands
Specific: 62 kDa
Positive control
10 Gy IR
Negative control
Mock IR
Additional data
Additional Notes
Lane 1: Untreated control
Lane 2: + 10 Gy IR (0 Minute)
Lane 3: + 10 Gy IR (30 Minute)
Lane 4: + 10 Gy IR (60 Minute)
Lane 2: + 10 Gy IR (0 Minute)
Lane 3: + 10 Gy IR (30 Minute)
Lane 4: + 10 Gy IR (60 Minute)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 13 2013