Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell line)
Loading amount
40 µg
Specification
HeLa cell line
Treatment
mitomycin C
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA+TTBS 1%
Secondary antibody
Name
Non-Abcam antibody was used: Goat polyclonal versus Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 57 kDa
Positive control
Lane 1: HeLa NT
Lane 2: HeLa T with 20 nM mitomycin C for 6 hours
Lane 3: HeLa T with 40 nM mitomycin C for 6 hours
Lane 2: HeLa T with 20 nM mitomycin C for 6 hours
Lane 3: HeLa T with 40 nM mitomycin C for 6 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 25 2012