Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Lung Epithelial Fibroblasts
Treatment
30GY Ionising Radiation
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Concentration
1 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 5% Milk in PBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP (DAKO)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~55kDa kDa
Positive control
MRC5VA exposed to 30Gy Ionising Radiation and left for 30 mins to initiate repair.
Negative control
MRC5VA un-irradiated
Additional data
Additional Notes
There is an increase in the signal from the un-IR to the IR samples which may not be as noticeable in the image as it is in the blot itself.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 13 2011