Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Kc167)
Loading amount
10 µg
Specification
Kc167
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA 0.1% TBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
Additional data
Additional Notes
Lanes 1-2, Kc167 cells in duplicate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Ravi Dashnamoorthy
Verified customer
提交于 Mar 24 2008