Application
Western blot
Sample
Human Cell lysate - whole cell (human breast adenocarcinoma cell line (MCF7))
Loading amount
35 µg
Specification
human breast adenocarcinoma cell line (MCF7)
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ecl+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 54 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 14 2011