Anti-Cdc37抗体(ab115166)
Key features and details
- Rabbit polyclonal to Cdc37
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cdc37抗体
参阅全部 Cdc37 一抗 -
描述
兔多克隆抗体to Cdc37 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide within Human Cdc37 aa 1-50. The exact sequence is proprietary.
Database link: Q16543 -
阳性对照
- Hela, 293T and mpuse NIH3T3 cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115166于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 44 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. -
序列相似性
Belongs to the CDC37 family. -
翻译后修饰
Constitutively sumoylated by UBE2I. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 101133417 Gorilla
- Entrez Gene: 11140 Human
- Entrez Gene: 12539 Mouse
- Entrez Gene: 114562 Rat
- Entrez Gene: 484955 Rhesus monkey
- Omim: 605065 Human
- SwissProt: Q16543 Human
- SwissProt: Q61081 Mouse
see all -
别名
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
see all
图片
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All lanes : Anti-Cdc37 antibody (ab115166) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Predicted band size: 44 kDa
Exposure time: 30 seconds -
Immunoprecipitation of Cdc37 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdc37 antibody (ab115166); Lane 2-Immunoprecipitation with an anti-Cdc37 antibody that recognizes an downstream epitope ; Lane 3- Immunoprecipitation with IgG control. Anti-Cdc37 antibody (ab115166) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab115166 尚未被引用在任何文献中。