概述

  • 产品名称
  • 描述
    兔多克隆抗体to Cdc27
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from the C-terminal region of Human Cdc27.

  • 阳性对照
    • Human Breast and Human Skin tissues, Mouse liver tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab113361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 92 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the APC3/CDC27 family.
    Contains 9 TPR repeats.
  • 翻译后修饰
    Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANAPC3 antibody
    • Anaphase Promoting Complex 3 antibody
    • Anaphase promoting complex protein 3 antibody
    • Anaphase Promoting Complex Subunit 3 antibody
    • Anaphase-promoting complex subunit 3 antibody
    • APC 3 antibody
    • APC3 antibody
    • Cdc 27 antibody
    • Cdc27 antibody
    • CDC27 homolog antibody
    • CDC27_HUMAN antibody
    • CDC27Hs antibody
    • Cell Division Cycle 27 antibody
    • Cell division cycle protein 27 homolog antibody
    • D0S1430E antibody
    • D17S978E antibody
    • H NUC antibody
    • H-NUC antibody
    • HNUC antibody
    • Nuc2 homolog antibody
    see all

图片

  • Lane 1 : Anti-Cdc27 antibody (ab113361) at 1 µg/ml
    Lane 2 : Anti-Cdc27 antibody (ab113361) at 2 µg/ml

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse liver tissue lysate


    Predicted band size : 92 kDa
  • Staining of Human Cdc27 in a Formalin/PFA-fixed paraffin-embedded section of Human Breast using ab113361 at a concentration of 5 µg/ml.
  • Staining of Human Cdc27 in a Formalin/PFA-fixed paraffin-embedded section of Human Skin using ab113361 at a concentration of 5 µg/ml.

文献

ab113361 has not yet been referenced specifically in any publications.

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