Application
Western blot
Sample
Cow Cell lysate - whole cell (Bovine Brain microvascular endothelial cells)
Loading amount
40 µg
Specification
Bovine Brain microvascular endothelial cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk 5% in TTBS 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase conjugated affiniPure Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
HRP substrate luminol
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 22 kDa
Additional data
Additional Notes
On the picture you can see the results obtained when using this antibody on bovine cells. Each band corresponds to a different treatment of cells (eg inflammation, hypoxia,...)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Cyril Mougin
Verified customer
提交于 Feb 11 2011