Application
Western blot
Sample
Human Tissue lysate - whole (Human breast tumour)
Specification
Human breast tumour
Treatment
2mg/kg doxorubicin and 0.1mg/kg zoledronic acid
Gel Running Conditions
Reduced Non-Denaturing (Native) (10 % polyacrylamide gel)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
Supersignal ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Penelope Ottewell
Verified customer
提交于 Jul 04 2008