Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Submitted composite image shows immunostaining results using ab32366 ( upper image) and also ab52295 ( lower image)
One would expect far less positivity in the lower image as ab52295 detects only cleaved caspase.
I am not familiar with the immunostaining pattern of pro Caspase 6 but, there appears to be a moderate cytoplasmic, diffuse positivity in most cells with an intense cytoplasmic positivity in some cells, most evident in the germinal centre/mantle zone.
Please compare positivity seen on tonsil by HPA:
http://www.proteinatlas.org/ENSG00000138794-CASP6/tissue/tonsil
Abcam response
Thank you very much for your data, we hope this is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Feb 13 2017