Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Embryo)
Permeabilization
Yes - 0.1%Tween
Specification
Embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PSB+10%FBS+0.2%BSA
Dilution
1/200
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Alexa Flour Goat anti-rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
This was a testing trial order with following discount code:
Abcam response
Thank you for testing this antibody in ICC/IF as part of our AbTrial program. We appreciate the feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Maneeshi Prasad
Verified customer
提交于 Oct 08 2015