Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle (AT))
Loading amount
50 µg
Specification
Skeletal muscle (AT)
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in TBS-Tween (0.05%)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 36, 20, 18 kDa Non-specific: 40, 48, 63, 75 kDa
Positive control
Lysate from mouse heart & Jurkat cell lysate.
Additional data
Additional Notes
Nitrocellulose membrane
1% SDS lysis buffer with 1:100 protease inhibitors
Lanes 1-8: Lysates from AT skeletal muscle
Lane 9: Lysate from mouse Liver
Lane 10: Jurkat cell lysate
1% SDS lysis buffer with 1:100 protease inhibitors
Lanes 1-8: Lysates from AT skeletal muscle
Lane 9: Lysate from mouse Liver
Lane 10: Jurkat cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. G.K Sakellariou
Verified customer
提交于 Sep 28 2012