Anti-Caspase-3抗体(ab13847)
Key features and details
- Rabbit polyclonal to Caspase-3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Caspase-3抗体
参阅全部 Caspase-3 一抗 -
描述
兔多克隆抗体to Caspase-3 -
宿主
Rabbit -
特异性
Stimulation is required to allow detection of the 17kDa cleaved form of the protein. Please see images below for recommended treatment conditions and positive controls.
ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells.
Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. We would recommend ab32351 and ab184787 as an alternative product for this application.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Dog, Pig, Xenopus laevis, Drosophila melanogaster, Indian muntjac, Zebrafish, Rhesus monkey, Chinese hamster, Common marmoset, Schmidtea mediterranea, Salvelinus alpinus -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Hap-1 WT, Human Caspase 3 (active) Recombinant Protein ICC: HeLa cells treated with 1 mM staurosporine
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13847于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (16) |
1/500. Detects a band of approximately 17, 34 kDa (predicted molecular weight: 17, 34 kDa).
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说明 |
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WB
1/500. Detects a band of approximately 17, 34 kDa (predicted molecular weight: 17, 34 kDa). |
靶标
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. -
组织特异性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
序列相似性
Belongs to the peptidase C14A family. -
翻译后修饰
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 836 Human
- Entrez Gene: 12367 Mouse
- Entrez Gene: 397244 Pig
- Entrez Gene: 25402 Rat
- Entrez Gene: 397720 Xenopus laevis
- Entrez Gene: 140621 Zebrafish
- Omim: 600636 Human
- SwissProt: P42574 Human
see all -
别名
- A830040C14Rik antibody
- Apopain antibody
- CASP 3 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate + Staurosporine (ab146588) (1μM for 4h)
Lane 2: Wild-type HAP1 cell lysate
Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (ab146588) (1μM for 4h)
Lane 4: Caspase-3 knockout HAP1 cell lysateLanes 1 - 4: Merged signal (red and green). Green - ab13847 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-Caspase-3 antibody (ab13847) at 1 µg/ml
Lane 1 : Human Caspase 3 (active) Recombinant Protein
Lane 2 : Human Pro Caspase 3 (inactive) Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17, 34 kDa
Observed band size: 17,32 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesCaspase 3 exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce large (17kDa) and small (12kDa) subunits. These subunits dimerize to form the active enzyme. ab13847 specifically detects the large active subunit (17kDa) and the inactive pro Caspase 3 (32 kDa).
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab13847 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Secondary antibody - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1118)
ab13847 被引用在 1118 文献中.
- Chen P et al. Metabolic adaptation to high-starch diet in largemouth bass (Micropterus salmoides) was associated with the restoration of metabolic functions via inflammation, bile acid synthesis and energy metabolism. Br J Nutr 129:381-394 (2023). PubMed: 35473811
- Kwesiga MP et al. Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment. Bioact Mater 23:261-273 (2023). PubMed: 36439083
- Qi C et al. LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4. Biol Direct 17:1 (2022). PubMed: 34991683
- An N et al. Inhibition of Rac1 attenuates radiation-induced lung injury while suppresses lung tumor in mice. Cell Death Discov 8:26 (2022). PubMed: 35031595
- Liu Y et al. Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma. Genes (Basel) 13:N/A (2022). PubMed: 35052471