Anti-Caspase-12抗体(ab62484)
Key features and details
- Rabbit polyclonal to Caspase-12
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Caspase-12抗体
参阅全部 Caspase-12 一抗 -
描述
兔多克隆抗体to Caspase-12 -
宿主
Rabbit -
特异性
This antibody reacts with both the propeptide and cleaved forms of Caspase-12.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
Database link: O08736 -
阳性对照
- WB: Human heart lysate. Rat dorsal root ganglion. IHC-P: Human and rat heart tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Caspase-12 Antibody is (Large)affinity chromatography purified via peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62484于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (6) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates. |
IHC-P | (1) |
Use a concentration of 2 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates. |
IHC-P
Use a concentration of 2 µg/ml. |
靶标
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功能
Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. -
组织特异性
Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. - Information by UniProt
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数据库链接
- Entrez Gene: 100506742 Human
- Entrez Gene: 156117 Rat
- Omim: 608633 Human
- SwissProt: Q6UXS9 Human
- SwissProt: Q920D5 Rat
- Unigene: 476989 Human
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别名
- CASP 12 antibody
- CASP-12 antibody
- Casp12 antibody
see all
图片
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Lane 1 : Anti-Caspase-12 antibody (ab62484) at 0.5 µg/ml
Lane 2 : Anti-Caspase-12 antibody (ab62484) at 1 µg/ml
Lane 3 : Anti-Caspase-12 antibody (ab62484) at 2 µg/ml
All lanes : Human heart lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit IgG secondary antibody
Predicted band size: 46 kDa
Observed band size: 46 kDa -
Immunohistochemical analysis of paraffin-embedded rat heart tissue stained for Caspase-12 using ab62484 at 2.5 µg/ml.
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All lanes : Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution
Lane 1 : Rat dorsal root ganglion
Lane 2 : Treated rat dorsal root ganglion
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit HRP-linked secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat -
Paraffin-embedded human heart tissue stained for Caspase-12 using ab62484 at 2 µg/ml dilution in immunohistochemical analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (115)
ab62484 被引用在 115 文献中.
- Desai VG et al. Doxorubicin-induced delayed-onset subclinical cardiotoxicity in mice. J Appl Toxicol 42:778-792 (2022). PubMed: 34668590
- Duan S et al. Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells. Ren Fail 44:126-136 (2022). PubMed: 35172689
- Meng S et al. CircAXL Knockdown Alleviates Aβ1-42-Induced Neurotoxicity in Alzheimer's Disease via Repressing PDE4A by Releasing miR-1306-5p. Neurochem Res 47:1707-1720 (2022). PubMed: 35229272
- Malik A et al. Interleukin-10 Mitigates Doxorubicin-Induced Endoplasmic Reticulum Stress as Well as Cardiomyopathy. Biomedicines 10:N/A (2022). PubMed: 35453640
- Zhang H et al. Rutaecarpine ameliorates lipopolysaccharide-induced BEAS-2B cell injury through inhibition of endoplasmic reticulum stress via activation of the AMPK/SIRT1 signaling pathway. Exp Ther Med 23:373 (2022). PubMed: 35495603