Anti-Caspase-10/CASP-10抗体(ab2012)
Key features and details
- Rabbit polyclonal to Caspase-10/CASP-10
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Caspase-10/CASP-10抗体
参阅全部 Caspase-10/CASP-10 一抗 -
描述
兔多克隆抗体to Caspase-10/CASP-10 -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Human -
免疫原
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阳性对照
- WB: HeLa whole cell lysate. ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
DEAE-Chromatography -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent concentration.
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|
WB |
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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说明 |
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ICC
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
靶标
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
-AMC and Asp-Glu-Val-Asp-
-AMC.
Isoform C is proteolytically inactive. -
组织特异性
Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. -
疾病相关
Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease. -
序列相似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
翻译后修饰
Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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数据库链接
- Entrez Gene: 843 Human
- Omim: 601762 Human
- SwissProt: Q92851 Human
- Unigene: 5353 Human
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别名
- ALPS2 antibody
- Apoptotic protease Mch-4 antibody
- CASP 10 antibody
see all
图片
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All lanes : Anti-Caspase-10/CASP-10 antibody (ab2012) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : K562 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Predicted band size: 59 kDa
Observed band size: 59 kDa -
Immunocytochemistry/Immunofluorescence of HeLa cells labeling Caspase-10/CASP-10 with Anti-Caspase-10/CASP-10 antibody (ab2012) at 5μg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab2012 被引用在 3 文献中.
- Langford MP et al. Multiple caspases mediate acute renal cell apoptosis induced by bacterial cell wall components. Ren Fail 33:192-206 (2011). PubMed: 21332342
- Pagnan G et al. The combined therapeutic effects of bortezomib and fenretinide on neuroblastoma cells involve endoplasmic reticulum stress response. Clin Cancer Res 15:1199-209 (2009). WB ; Human . PubMed: 19228726
- Green KA et al. Caspase-mediated Cleavage of Insulin Receptor Substrate. J Biol Chem 279:25149-56 (2004). PubMed: 15069074