Anti-Casein Kinase 2 beta (phospho S209)抗体(ab12861)
Key features and details
- Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Casein Kinase 2 beta (phospho S209)抗体
参阅全部 Casein Kinase 2 beta 一抗 -
描述
兔多克隆抗体to Casein Kinase 2 beta (phospho S209) -
宿主
Rabbit -
特异性
Does not cross react with Casein Kinase 2 alpha [pS360] (mouse), [pS362], [pS370], or Casein Kinase 2 beta pS2]. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cell lysates or K562 cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12861于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
说明 |
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Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit. -
序列相似性
Belongs to the casein kinase 2 subunit beta family. -
翻译后修饰
Phosphorylated by alpha subunit. - Information by UniProt
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数据库链接
- Entrez Gene: 1460 Human
- Omim: 115441 Human
- SwissProt: P67870 Human
- Unigene: 73527 Human
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别名
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
see all
图片
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Peptide Competition and Phosphatase Treatment
Lysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with ab12861 antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method.The data show that only the peptide corresponding to ab12861 blocks the signal, verifying the specificity of the antibody.
Peptide Competition and Phosphatase Treatment
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab12861 尚未被引用在任何文献中。