Anti-Cardiac Troponin T抗体[1F11] (ab10214)
Key features and details
- Mouse monoclonal [1F11] to Cardiac Troponin T
- Suitable for: Sandwich ELISA, ICC
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
概述
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产品名称
Anti-Cardiac Troponin T抗体[1F11]
参阅全部 Cardiac Troponin T 一抗 -
描述
小鼠单克隆抗体[1F11] to Cardiac Troponin T -
宿主
Mouse -
特异性
Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813) -
经测试应用
适用于: Sandwich ELISA, ICCmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Tissue, cells or virus corresponding to Human Cardiac Troponin T. Free human cardiac troponin T
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表位
Peptide 146-160. -
阳性对照
- ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)
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常规说明
This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Concentration varies from lot to lot and can be provided on request.
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purity tested by electrophoresis. -
Primary antibody说明
Does not cross-react with skeletal muscle troponin T, cTnI or TnC. -
克隆
单克隆 -
克隆编号
1F11 -
骨髓瘤
Sp2/0 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10214于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
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ICC | (2) |
Use a concentration of 1 - 5 µg/ml.
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说明 |
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Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection. |
ICC
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
组织特异性
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
疾病相关
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
序列相似性
Belongs to the troponin T family. - Information by UniProt
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数据库链接
- Entrez Gene: 7139 Human
- Omim: 191045 Human
- SwissProt: P45379 Human
- Unigene: 533613 Human
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别名
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
图片
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Immunofluorescence staining of Cardiac Troponin T using ab10214 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab10214 at 1 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab10214 also gave a positive staining using 4% formaldehyde fixation (10 min).
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Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5µg/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (59)
ab10214 被引用在 59 文献中.
- Haryono A et al. ChGn-2 Plays a Cardioprotective Role in Heart Failure Caused by Acute Pressure Overload. J Am Heart Assoc 11:e023401 (2022). PubMed: 35322673
- Li X et al. Oxidative Stress Contributes to Cytoskeletal Protein Degradation of Esox lucius through Activation of Mitochondrial Apoptosis during Postmortem Storage. Foods 11:N/A (2022). PubMed: 35564031
- He Y et al. A smart adhesive Janus hydrogel for non-invasive cardiac repair and tissue adhesion prevention. Nat Commun 13:7666 (2022). PubMed: 36509756
- Wang Q et al. Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells. Sci China Life Sci 64:269-281 (2021). PubMed: 32712833
- Pierre M et al. NaV1.5 knockout in iPSCs: a novel approach to study NaV1.5 variants in a human cardiomyocyte environment. Sci Rep 11:17168 (2021). PubMed: 34433864