Application
Western blot
Sample
Human Cell lysate - whole cell (Human cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Loading amount
20 µg
Specification
Human cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: anti-rabbit HRP conjugation HRP
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce pico ecl kit
Exposure
30 second(s)
Bands
Specific: 15 kDa
Positive control
Observed a band around 15 kDa for human cardiomyoctes,however a band was also seen for mouse cardiomyocytes at around 10 kDa. No band was observed for rat cardiomyoctes or the HC92 control cell line used.
Additional data
Abcam response
Abcam has not tested ab45966 in rat cardiomyocytes or HC92 cells and your data suggest it may not be suitable for this species using the present protocol. Since the molecular weight of Cardiac FABP is 15 kDa we suggest using a gel with a mono/bis percentage of 12% to 16%. Many thanks for your feedback on this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Prof. Paul Townsend
Verified customer
提交于 Mar 18 2010