Anti-Carbonic Anhydrase 9/CA9抗体(ab15086)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 9/CA9
- Suitable for: Flow Cyt (Intra), ICC, IHC-Fr, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Carbonic Anhydrase 9/CA9抗体
参阅全部 Carbonic Anhydrase 9/CA9 一抗 -
描述
兔多克隆抗体to Carbonic Anhydrase 9/CA9 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC, IHC-Fr, IHC-P, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
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阳性对照
- WB: Ramos, A549, HeLa and MDA-MB-231 cell lysates; Rat renal cortex lysate. Intr Flow Cyt: A-431 and U-87 MG cells. ICC: A-431 cells. IHC-Fr: Human renal cell cancer tumor sections. IHC-P: Human breast cancer sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15086于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/1000.
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ICC |
Use a concentration of 2 - 5 µg/ml.
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IHC-Fr | (1) |
1/200 - 1/500.
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IHC-P | (2) |
1/200 - 1/500.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49.7 kDa).Can be blocked with Recombinant Human RND1 protein (ab109840).
|
说明 |
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Flow Cyt (Intra)
1/1000. |
ICC
Use a concentration of 2 - 5 µg/ml. |
IHC-Fr
1/200 - 1/500. |
IHC-P
1/200 - 1/500. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49.7 kDa).Can be blocked with Recombinant Human RND1 protein (ab109840). |
靶标
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功能
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
组织特异性
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
序列相似性
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
翻译后修饰
Asn-346 bears high-mannose type glycan structures. -
细胞定位
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 768 Human
- Entrez Gene: 313495 Rat
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
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别名
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
see all
图片
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Western blot analysis labeling Carbonic Anhydrase 9/CA9 with ab15086 at 1 ug/ml in rat renal cortex lysate.
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Western blot analysis labeling Carbonic Anhydrase 9/CA9 with ab15086 at 1 ug/ml in 1) HeLa, 2) MDA-MB-231, and 3) A549 whole cell lysates.
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Immunohistochemistry analysis of a formalin-fixed, paraffin-embedded tissue section of human breast cancer using ab15086 at 1:1000 dilution. The primary antibody bound to Carbonic Anydrase 9/CA9 antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This antibody generated an expected cytoplasmic staining of Carbonic Anydrase 9/CA9 protein with an intense signal around the cellular membranes in tumor cores. The latter are more likely to be hypoxic in growing tumors which signifies that the observed Carbonic Anydrase 9/CA9 staining is specific.
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Immunohistochemistry analysis of human renal cell cancer tumor cryosections stained with ab15086 at a 1/500 dilution (left) or normal rabbit serum (right).
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All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086) at 1 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 49.7 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (134)
ab15086 被引用在 134 文献中.
- Zatovicova M et al. ADAM10 mediates shedding of carbonic anhydrase IX ectodomain non‑redundantly to ADAM17. Oncol Rep 49:N/A (2023). PubMed: 36524367
- Hamon P et al. TGFβ receptor inhibition unleashes interferon-β production by tumor-associated macrophages and enhances radiotherapy efficacy. J Immunother Cancer 10:N/A (2022). PubMed: 35301235
- Lu C et al. Hypoxia-activated neuropeptide Y/Y5 receptor/RhoA pathway triggers chromosomal instability and bone metastasis in Ewing sarcoma. Nat Commun 13:2323 (2022). PubMed: 35484119
- Gombodorj N et al. Effects of Ultrafine Single-Nanometer Oxygen Bubbles on Radiation Sensitivity in a Tumor-Bearing Mouse Model. Int J Mol Sci 23:N/A (2022). PubMed: 35743281
- Takei J et al. Impact of Neoadjuvant Bevacizumab on Neuroradiographic Response and Histological Findings Related to Tumor Stemness and the Hypoxic Tumor Microenvironment in Glioblastoma: Paired Comparison Between Newly Diagnosed and Recurrent Glioblastomas. Front Oncol 12:898614 (2022). PubMed: 35785200