Application
Western blot
Sample
Mouse Cell lysate - whole cell (skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% Milk + TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Mouse anti - rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
3 second(s)
Bands
Specific: 60 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 17 2008