Application
Flow Cytometry
Sample
Sacrophilus harrisii (Tasmanian Devil) Cell (C5065 (tasmanian devil tumour cell line))
Permeabilization
Yes - PBS/0.1% tween
Gating Strategy
Population was gated for single cells and DAPI used to exclude dead cells.
Specification
C5065 (tasmanian devil tumour cell line)
Preparation
Cell harvesting/tissue preparation method: Cells were washed in PBS counted and resuspended at 5x10/6 cells per ml in FACS buffer. 100ul was added to tubes for fixation, permeabilisation and staining.
Sample buffer: FACS buffer (PBS/0.5% BSA/0.05% azide)
Sample buffer: FACS buffer (PBS/0.5% BSA/0.05% azide)
Fixation
80% methanol
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: FACS buffer
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Cells were fixed in ice cold 80% methanol for 5 minutes, washed then permeabilised in PBS/0.1% tween for 20 minutes. Washed then incubated in 0.3M glycine/PBS/10% normal goat serum for 20 minutes to block, then washed and proceed to antibody staining. Isotope control was rabbit IgG (non abcam antibody)
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提交于 Dec 01 2016