Application
Immunocytochemistry
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti Rabbit IgG (H+L)
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mahesh Shivananjappa
Verified customer
提交于 Feb 02 2012