Application
Western blot
Sample
Mouse Tissue lysate - other (microsomes)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
3 µg
Specification
microsomes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS-T
Dilution
1/4000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 75 kDa
Positive control
microsomal preps from liver tissue
Additional data
Additional Notes
The lower band is my gene of interest and the upper band is calnexin, which served as my loading control. What really amazes me is that at 1:4000 initial dilution, I have been able to reuse this primary antibody 7 times! Developing time is 2 minutes but exposure is 1 second.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Arlene Lim
Verified customer
提交于 Aug 30 2013