Anti-Calnexin抗体(ab13505)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Calnexin抗体
参阅全部 Calnexin 一抗 -
描述
兔多克隆抗体to Calnexin -
宿主
Rabbit -
特异性
Recognizes ER membrane, mitochondria and cis-Golgi -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide:
TAPPSSPKVTYKAPVPTGE
conjugated to KLH, corresponding to amino acids 50-68 of Human Calnexin -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13505于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100.
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ICC/IF |
1/100.
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WB |
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
|
说明 |
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IHC-P
1/100. |
ICC/IF
1/100. |
WB
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
靶标
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功能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
序列相似性
Belongs to the calreticulin family. -
细胞定位
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- SwissProt: P35565 Rat
- Unigene: 567968 Human
see all -
别名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
图片
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Anti-Calnexin antibody (ab13505) at 1/1000 dilution + Mixture of tissue lysates prepared from Rat tissues.
Predicted band size: 90 kDa -
ab13505 staining Calnexin - ER membrane marker in mouse back skin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. The primary antibody was used at 1/100 dilution and a Fluorophore conjugated goat anti rabbit secondary at 1/50 dilution. 
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ab13505 staining Calnexin - ER membrane marker in mouse colitis colon tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated anti rabbit was used at 1/10 dilution as secondary.
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Anti-Calnexin antibody (ab13505) at 1/2000 dilution ((in TBS with 0.05% TW-20)) + HeLa cell lysate
Secondary
An HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 90 kDa -
ab13505 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab13505 被引用在 4 文献中.
- Weghorst F et al. Caspase-3 Cleaves Extracellular Vesicle Proteins During Auditory Brainstem Development. Front Cell Neurosci 14:573345 (2020). PubMed: 33281555
- Rennoll-Bankert KE et al. Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion. PLoS Pathog 11:e1005115 (2015). PubMed: 26291822
- Guziewicz KE et al. Molecular consequences of BEST1 gene mutations in canine multifocal retinopathy predict functional implications for human bestrophinopathies. Invest Ophthalmol Vis Sci 52:4497-505 (2011). PubMed: 21498618
- Grimsby JL et al. Role of lysyl oxidase propeptide in secretion and enzyme activity. J Cell Biochem 111:1231-43 (2010). WB . PubMed: 20717923