Application
Western blot
Sample
Chinese Hamster Cell lysate - other (CHO cells)
Loading amount
15 µg
Specification
CHO cells
Gel Running Conditions
Reduced Denaturing (7.5 % Tris-Gly)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/4000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 % milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated Goat anti-Rabbit Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~90 kDa
Additional data
Additional Notes
mF = membrane fraction (100,000 x g pellet)
CF= cytosolar fraction
CF= cytosolar fraction
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Yannick Waumans
Verified customer
提交于 Jun 29 2011