重组Anti-Calmodulin 1/2/3抗体[EP799Y] - C-terminal (ab45689)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP799Y] to Calmodulin 1/2/3 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Calmodulin 1/2/3抗体[EP799Y] - C-terminal
参阅全部 Calmodulin 1/2/3 一抗 -
描述
兔单克隆抗体[EP799Y] to Calmodulin 1/2/3 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, IP, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Calmodulin 1/2/3 aa 100 to the C-terminus. The exact sequence is proprietary.
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阳性对照
- WB: C6, RAW264.7, NIH/3T3, HeLa, HCT 116 cell lysate. IHC-P: Human urinary bladder carcinoma and testis tissues. Flow Cyt (intra): MCF7 and NIH/3T3 cells. IP: Human skeletal muscle tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP799Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45689于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/50 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (10) |
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa).
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Mouse and rat species reactivity is recommended based on WB results, we do not guarantee IHC for mouse or rat. |
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IP | (1) |
1/20 - 1/50.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
Mouse and rat species reactivity is recommended based on WB results, we do not guarantee IHC for mouse or rat. |
说明 |
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Flow Cyt (Intra)
1/50 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Mouse and rat species reactivity is recommended based on WB results, we do not guarantee IHC for mouse or rat. |
IP
1/20 - 1/50. |
IHC-Fr
Use at an assay dependent concentration. Mouse and rat species reactivity is recommended based on WB results, we do not guarantee IHC for mouse or rat. |
靶标
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相关性
Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. -
细胞定位
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. -
数据库链接
- Entrez Gene: 808 Human
- Entrez Gene: 12315 Mouse
- Entrez Gene: 24244 Rat
- Omim: 114182 Human
- Omim: 114183 Human
- SwissProt: 114180 Human
- SwissProt: P0DP23 Human
- SwissProt: P0DP24 Human
see all -
形式
There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. -
别名
- CALM 1 antibody
- CALM 2 antibody
- CALM 3 antibody
see all
图片
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ΔSRCR peripheral blood monocyte-derived macrophages (PMMs) still function as hemoglobin-haptoglobin (Hb-Hp) scavengers.
Porcine PMMs were incubated for 24 hours in the presence of 100 μg/mol Hb-Hp then lysed with reducing SDS sample buffer and HO-1 protein expression analyzed by western blot.
For full details please see paper.
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All lanes : Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal (ab45689) at 1/10000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma) whole cell lysate
Blocking peptides at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDaBlocking and Diluting buffer: 5% NFDM/TBST
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ab45689 immunoprecipitating Calmodulin. 10 µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: Human skeletal muscle lysate (10 µg)
Lane 2: Human skeletal muscle lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45689 in human skeletal muscle lysate -
ab45689 staining Calmodulin in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control: PBS in place of primary antibody (inset).
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ab45689 at a 1:250 dilution staining Calmodulin in human urinary bladder carcinoma tissue by immunohistochemistry in paraffin embedded tissue.
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All lanes : Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal (ab45689) at 1/10000 dilution
Lane 1 : RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
Lane 2 : C6 (rat glioma) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal (ab45689) at 1/5000 dilution + NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 10 µg
Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted? -
Overlay histogram showing MCF7 (Human breast adenocarcinoma cell line) cells stained with ab45689 (red line).
The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45689, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C.
Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a decreased signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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ab45689 staining Calmodulin in NIH/3T3 (Mouse embryo fibroblast cell line) cells by intracellular flow cytometry.
Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabeled control: Cell without incubation with primary antibody and secondary antibody (Blue)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (54)
ab45689 被引用在 54 文献中.
- Costa-da-Silva AC et al. Salivary ZG16B expression loss follows exocrine gland dysfunction related to oral chronic graft-versus-host disease. iScience 25:103592 (2022). PubMed: 35005541
- Wang XR et al. Mesenteric Lymph Drainage Improves Cardiac Papillary Contractility and Calcium Sensitivity in Rats with Hemorrhagic Shock. J Surg Res 266:245-253 (2021). PubMed: 34034059
- Vuong-Brender TT et al. Neuronal calmodulin levels are controlled by CAMTA transcription factors. Elife 10:N/A (2021). PubMed: 34499028
- Nejedlá M et al. The actin regulator profilin 1 is functionally associated with the mammalian centrosome. Life Sci Alliance 4:N/A (2021). PubMed: 33184056
- Sjøgaard-Frich LM et al. Dynamic Na+/H+ exchanger 1 (NHE1) - calmodulin complexes of varying stoichiometry and structure regulate Ca2+-dependent NHE1 activation. Elife 10:N/A (2021). PubMed: 33655882