Anti-Caldesmon/CDM抗体(ab68878)
Key features and details
- Rabbit polyclonal to Caldesmon/CDM
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Caldesmon/CDM抗体
参阅全部 Caldesmon/CDM 一抗 -
描述
兔多克隆抗体to Caldesmon/CDM -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Cow, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human Caldesmon/CDM aa 750 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab86635) -
阳性对照
- This antibody gave a positive signal in SK N SH Whole Cell Lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68878于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 93 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 93 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. -
组织特异性
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. -
序列相似性
Belongs to the caldesmon family. -
结构域
The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form. -
翻译后修饰
In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). - Information by UniProt
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数据库链接
- Entrez Gene: 472523 Chimpanzee
- Entrez Gene: 281660 Cow
- Entrez Gene: 800 Human
- Entrez Gene: 109624 Mouse
- Entrez Gene: 100009077 Rabbit
- Entrez Gene: 25687 Rat
- Entrez Gene: 707050 Rhesus monkey
- Omim: 114213 Human
see all -
别名
- CAD antibody
- CALD 1 antibody
- CALD1 antibody
see all
图片
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All lanes : Anti-Caldesmon/CDM antibody (ab68878) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?The band seen at 78 kDa is consistent with the banding pattern observed for other commercially available antibodies to Caldesmon/CDM.
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ICC/IF image of ab68878 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68878, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2, Hek293 and MCF7 cells at 5µg/ml.
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IHC image of Caldesmon/CDM staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68878 at 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab68878 被引用在 3 文献中.
- Kuo CH et al. Effects of varying gelatin coating concentrations on RANKL induced osteoclastogenesis. Exp Cell Res 400:112509 (2021). PubMed: 33529711
- Ouyang X et al. In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves. J Peripher Nerv Syst 24:195-206 (2019). PubMed: 31119823
- Palladino SP et al. The Human Blood-Nerve Barrier Transcriptome. Sci Rep 7:17477 (2017). Human . PubMed: 29234067