Application
Western blot
Sample
Rat Tissue lysate - whole (Rat forebrain or cerebellar homogenates)
Specification
Rat forebrain or cerebellar homogenates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Dako P0399
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: ~130 kDa
Positive control
Rat forebrain homogenate is a suitable positive control
Additional data
Additional Notes
Incubation with antibody was performed at 4 degrees overnight. Bands were usually visible after 30 seconds-1 minute exposure with normal ECL when 15ug of protein was run on the gel. For some samples a longer exposure was required or a short exposure with advanced ECL.
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提交于 Aug 17 2005