Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%Milk
Secondary antibody
Name
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 8 kDa Non-specific: 13, 18, 67, 75 kDa
Additional data
Additional Notes
The target is partial recombinant CALCA protein, and expressed in E.coil.
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提交于 Aug 25 2010