Application
Western blot
Sample
Dog Cell lysate - whole cell (hOCT2/hMATE1 double transfected MDCK cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
hOCT2/hMATE1 double transfected MDCK cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% Non-Fat Milk
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)–Peroxidase antibo
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL (Thermo Scientific SuperSignal West
Negative control
None
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 39, 47, 70, 80, 100, 120 kDa
Positive control
On a different blot, tested same samples with mouse kidney lysate and got bands around 30 kDa
Additional data
Additional Notes
Lane 1: Molecular weight marker (magic marker)
Lane 2: HK-2 whole cell lysate - no treatment
Lane 3: HK-2 whole cell lysate - no treatment
Lane 4: HK-2 whole cell lysate - no treatment
Lane 5: RPTEC whole cell lysate - no treatment
Lane 6: Empty vector MDCK - whole cell lysate - no treatment
Lane 7: hOCT2/hMATE1 overexpressing MDCK - whole cell lysate - no treatment
Lane 8: Empty vector HEK293-Flp-In - whole cell lysate - no treatment
Lane 9: OCT2 overexpressing HEK293-Flp-In - whole cell lysate - no treatment
Lane 10: MATE1 overexpressing HEK293-Flp-In - whole cell lysate - no treatment
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 10 2017