Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain section)
Specification
Brain section
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/200
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbitAlexa 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
The mouse tissues were fixed (animals perfused with 4% PFA) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut with microtome (25 µm thickness). Immunofuorescent staining performed on mouse brain using the anti-Calbindin (ab 49899 ) with Alexa 488. The antibody stains neurons, calbindin stained the ER. Nucleus stained with Hoechst at high power magnification.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 May 20 2011