Anti-Calbindin抗体(ab49899)
Key features and details
- Rabbit polyclonal to Calbindin
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Calbindin抗体
参阅全部 Calbindin 一抗 -
描述
兔多克隆抗体to Calbindin -
宿主
Rabbit -
特异性
We recommend the use of RabMAb ab108404 for testing in IHC and ICC/IF for this target. -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Hamster, Cow, Pig -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Calbindin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49899于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa).
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说明 |
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IP
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa). |
靶标
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功能
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. -
序列相似性
Belongs to the calbindin family.
Contains 5 EF-hand domains. -
结构域
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium. - Information by UniProt
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数据库链接
- Entrez Gene: 539930 Cow
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 100153645 Pig
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P04467 Cow
- SwissProt: P05937 Human
see all -
别名
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
图片
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All lanes : ab49899 at 1 µg/ml
Lane 1 : Brain (human) tissue lysate
Lane 2 : Brain (Mouse) Tissue lysate
Lane 3 : Brain (Rat) Tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 37 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
All lanes : Anti-Calbindin antibody (ab49899) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands. -
Calbindin - Neuronal Marker was immunoprecipitated using 0.5mg Mouse kidney whole tissue extract, 5µg of Rabbit polyclonal to Calbindin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse kidney whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49899.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 30kDa: Calbindin - Neuronal Marker.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab49899 被引用在 7 文献中.
- George B et al. Regulation of renal calbindin expression during cisplatin-induced kidney injury. J Biochem Mol Toxicol 36:e23068 (2022). PubMed: 35403300
- Ilnytska O et al. Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage. J Biol Chem 297:100813 (2021). PubMed: 34023384
- Pagliaro AH et al. Hemispheric asymmetry of calbindin-positive neurons is associated with successful song imitation. Brain Res 1732:146679 (2020). PubMed: 31981678
- Liu R et al. PDK1 Regulates the Maintenance of Cell Body and the Development of Dendrites of Purkinje Cells by pS6 and PKC?. J Neurosci 40:5531-5548 (2020). PubMed: 32487697
- Han TU et al. Human GNPTAB stuttering mutations engineered into mice cause vocalization deficits and astrocyte pathology in the corpus callosum. Proc Natl Acad Sci U S A 116:17515-17524 (2019). PubMed: 31405983
- Schludi MH et al. Spinal poly-GA inclusions in a C9orf72 mouse model trigger motor deficits and inflammation without neuron loss. Acta Neuropathol 134:241-254 (2017). PubMed: 28409281
- Schmouth JF et al. Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice. BMC Biol 11:106 (2013). IHC . PubMed: 24124870