Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Retina)
Permeabilization
Yes - Triton X-100 in Blocking Solution
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Davidson's Fixative
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Donkey Serum, BSA, Triton X-100, PBS
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Additional data
Additional Notes
The mole-rat retina does not show the rodent typical calbindin staining, but this is likely to be due to the retinal physiology of the subterranean retina.
Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in Fukomys anselli, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in Fukomys anselli which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 28 2017