Application
Immunohistochemistry (Frozen sections)
Sample
Cat Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/200
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocker
Secondary antibody
Name
Non-Abcam antibody was used: Cy3 DK anti-Goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Did not work for the staining of the frozen senctions.
Abcam response
Thank you very much for your valuable feedback.
We have not previously tested this antibody with cat samples or with frozen tissue, so it is possible that the antibody is not suitable for this application or species.
The reviewer was given a testing discount code to try this antibody in this assay.
We have not previously tested this antibody with cat samples or with frozen tissue, so it is possible that the antibody is not suitable for this application or species.
The reviewer was given a testing discount code to try this antibody in this assay.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 13 2012