Anti-CYFIP1抗体(ab108220)
Key features and details
- Rabbit polyclonal to CYFIP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-CYFIP1抗体
参阅全部 CYFIP1 一抗 -
描述
兔多克隆抗体to CYFIP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Lizard, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 1203 and 1253 of Human CYFIP1 (NP_055423.1).
-
阳性对照
- HeLa and 293T whole cell lysates
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab108220 was affinity purified using an epitope specific to CYFIP1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108220于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 145 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
说明 |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 145 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
-
功能
Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers. -
序列相似性
Belongs to the CYFIP family. -
细胞定位
Cytoplasm > perinuclear region. Cell projection > lamellipodium. Cell projection > ruffle. Cell junction > synapse > synaptosome. Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia. - Information by UniProt
-
数据库链接
- Entrez Gene: 23191 Human
- Omim: 606322 Human
- SwissProt: Q7L576 Human
- Unigene: 26704 Human
-
别名
- CY antibody
- CYFIP 1 antibody
- CYFIP1 antibody
see all
图片
-
All lanes : Anti-CYFIP1 antibody (ab108220) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
at 50 µg
Lane 2 : HeLa whole cell lysate
at 15 µg
Lane 3 : HeLa whole cell lysate
at 5 µg
Lane 4 : 293T whole cell lysate
at 50 µg
Developed using the ECL technique.
Predicted band size: 145 kDa
Exposure time: 3 minutes -
ab108220 at 1 µg/ml detecting CYFIP1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of CYFIP1
Lane 2: ab108220 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab108220 被引用在 2 文献中.
- Cioni JM et al. Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function. Neuron 97:1078-1093.e6 (2018). PubMed: 29518358
- Han K et al. Fragile X-like behaviors and abnormal cortical dendritic spines in cytoplasmic FMR1-interacting protein 2-mutant mice. Hum Mol Genet 24:1813-23 (2015). PubMed: 25432536