Anti-CXCL2抗体(ab9950)
Key features and details
- Rabbit polyclonal to CXCL2
- Suitable for: Sandwich ELISA, Neutralising, WB, ELISA
- Reacts with: Mouse
- Isotype: unknown
概述
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产品名称
Anti-CXCL2抗体
参阅全部 CXCL2 一抗 -
描述
兔多克隆抗体to CXCL2 -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISA, Neutralising, WB, ELISAmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant full length protein corresponding to Mouse CXCL2. Highly pure (>98%) recombinant mMIP-2 (mouse Macrophage Inflammatory Protein-2)
Database link: P10889 -
阳性对照
- Recombinant mouse CXCL2 protein (ab9952) can be used as a positive control in WB.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
|
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Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-2 (15 ng/ml), a concentration of 0.52 - 0.9 µg/ml of this antibody is required. |
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
ELISA |
Use at an assay dependent concentration.
To detect mMIP-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-2. |
说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-2 (15 ng/ml), a concentration of 0.52 - 0.9 µg/ml of this antibody is required. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ELISA
Use at an assay dependent concentration. To detect mMIP-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-2. |
靶标
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功能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 20310 Mouse
- SwissProt: P10889 Mouse
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别名
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
see all
数据表及文件
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Datasheet download
文献 (5)
ab9950 被引用在 5 文献中.
- Chen X et al. Anti-Semaphorin-7A single chain antibody demonstrates beneficial effects on pulmonary inflammation during acute lung injury. Exp Ther Med 15:2356-2364 (2018). PubMed: 29456642
- Zhang Q et al. Toll-like receptor 4 contributes to acute kidney injury after cardiopulmonary resuscitation in mice. Mol Med Rep 14:2983-90 (2016). Mouse . PubMed: 27510583
- Greer A et al. Site-Specific Neutrophil Migration and CXCL2 Expression in Periodontal Tissue. J Dent Res 95:946-52 (2016). PubMed: 27013641
- Zenobia C et al. Commensal bacteria-dependent select expression of CXCL2 contributes to periodontal tissue homeostasis. Cell Microbiol 15:1419-26 (2013). PubMed: 23433011
- Oue E et al. CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction. Biochem Biophys Res Commun 424:456-61 (2012). Neutralising . PubMed: 22771802