Anti-MED26抗体[2216C1a] (ab50619)
Key features and details
- Mouse monoclonal [2216C1a] to MED26
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-MED26抗体[2216C1a] -
描述
小鼠单克隆抗体[2216C1a] to MED26 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human MED26.
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阳性对照
- Recombinant MED26 fragment.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
ab50619 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Sterile filtered through a 0.22 micrometer membrane. -
克隆
单克隆 -
克隆编号
2216C1a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50619于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa). |
靶标
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功能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
序列相似性
Belongs to the Mediator complex subunit 26 family.
Contains 1 TFIIS N-terminal domain. -
细胞定位
Nucleus. - Information by UniProt
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别名
- Activator recruited cofactor 70 kDa component antibody
- Activator-recruited cofactor 70 kDa component antibody
- ARC 70 antibody
see all
图片
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Anti-MED26 antibody [2216C1a] (ab50619) + immunized recombinant MED26 fragment
Predicted band size: 65 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Note: actual size differs from predicted size as this blot was loaded with MED26 fragment.
数据表及文件
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Datasheet download
文献 (3)
ab50619 被引用在 3 文献中.
- Sato S et al. Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme. J Biol Chem 291:26886-26898 (2016). PubMed: 27821593
- Fowler T et al. Regulation of MYC expression and differential JQ1 sensitivity in cancer cells. PLoS One 9:e87003 (2014). ChIP ; Human . PubMed: 24466310
- Miotto B & Struhl K HBO1 histone acetylase activity is essential for DNA replication licensing and inhibited by Geminin. Mol Cell 37:57-66 (2010). PubMed: 20129055