Anti-Rootletin抗体(ab121653)
Key features and details
- Rabbit polyclonal to Rootletin
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Rootletin抗体 -
描述
兔多克隆抗体to Rootletin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICCmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Rootletin aa 406-483.
Sequence:VKRLEKQNLEKDQVNKDLTEKLEALESLRLQEQAALETEDGEGLQQTLRD LAQAVLSDSESGVQLSGSERTADASNGS
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阳性对照
- IHC-P: Human fallopian tube, bronchus, liver, cerebral cortex and testis tissue; ICC: U2OS cells; WB: SCLC-21H.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121653于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 229 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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说明 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 229 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
ICC
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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功能
Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis. -
序列相似性
Belongs to the rootletin family. -
翻译后修饰
Phosphorylated by NEK2 which may regulate its association with centrosomes. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytoskeleton > centrosome > centriole. In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 9696 Human
- SwissProt: Q5TZA2 Human
- Unigene: 309403 Human
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别名
- ciliary rootlet coiled coil antibody
- Ciliary rootlet coiled-coil protein antibody
- Crocc antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchus tissue labelling Rootletin with ab121653 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labelling Rootletin with ab121653 at 2 µg/mL.
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Anti-Rootletin antibody (ab121653) at 0.4 µg/ml + SCLC-21H (human small cell lung carcinoma cell line) cell lysate
Predicted band size: 229 kDa -
ab121653, at 1/150 dilution, staining Rootletin in Human fallopian tube tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
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Immunohistochemical analysis of human testis tissue labeling Rootletin with ab121653 at 1/50 dilution.
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Immunohistochemical analysis of human liver tissue labeling Rootletin with ab121653 at 1/50 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121653 被引用在 1 文献中.
- Saade M et al. Multimerization of Zika Virus-NS5 Causes Ciliopathy and Forces Premature Neurogenesis. Cell Stem Cell 27:920-936.e8 (2020). PubMed: 33147489