Anti-CRMP2抗体[1B1] (ab62539)
Key features and details
- Mouse monoclonal [1B1] to CRMP2
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-CRMP2抗体[1B1]
参阅全部 CRMP2 一抗 -
描述
小鼠单克隆抗体[1B1] to CRMP2 -
宿主
Mouse -
特异性
Does not react with CRMP-1, CRMP-3, CRMP-4 or CRMP-5.
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经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human CRMP2 (C terminal). A 15 residue peptide from C-terminal domain.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 1% Sucrose, 1.21% Tris, 0.375% Glycine -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1B1 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62539于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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说明 |
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Flow Cyt
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
靶标
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功能
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
翻译后修饰
3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1808 Human
- Entrez Gene: 12934 Mouse
- Omim: 602463 Human
- SwissProt: Q16555 Human
- SwissProt: O08553 Mouse
- Unigene: 173381 Human
- Unigene: 352648 Mouse
- Unigene: 475100 Mouse
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别名
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
see all
图片
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Anti-CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Overlay histogram showing SH-SY5Y cells stained with ab62539 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab62539 被引用在 3 文献中.
- Paskus JD et al. Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF Protein-Dependent Neuroligin-1 Mechanism of Action. Cell Rep 29:2944-2952.e5 (2019). PubMed: 31801062
- Zhou K et al. NMDA receptor hypofunction induces dysfunctions of energy metabolism and semaphorin signaling in rats: a synaptic proteome study. Schizophr Bull 38:579-91 (2012). WB ; Rat . PubMed: 21084551
- Chi XX et al. Regulation of N-type voltage-gated calcium channels (Cav2.2) and transmitter release by collapsin response mediator protein-2 (CRMP-2) in sensory neurons. J Cell Sci 122:4351-62 (2009). PubMed: 19903690