Anti-CRM1抗体(ab84375)
Key features and details
- Rabbit polyclonal to CRM1
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CRM1抗体
参阅全部 CRM1 一抗 -
描述
兔多克隆抗体to CRM1 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region within C terminal amino acids 1025-1071 (FAGEDTSDLF LEEREIALRQ ADEEKHKRQM SVPGIFNPHE IPEEMCD) of human CRM1 (NP_003391.1).
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阳性对照
- Human breast tumor; mouse squamous cell carcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified using an epitope specific to CRM1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84375于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
说明 |
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Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. -
组织特异性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney. -
序列相似性
Belongs to the exportin family.
Contains 10 HEAT repeats.
Contains 1 importin N-terminal domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 459534 Chimpanzee
- Entrez Gene: 7514 Human
- Entrez Gene: 103573 Mouse
- Entrez Gene: 718063 Rhesus monkey
- Omim: 602559 Human
- SwissProt: O14980 Human
- SwissProt: Q6P5F9 Mouse
- Unigene: 370770 Human
see all -
别名
- Chromosome region maintenance 1 protein homolog antibody
- CRM 1 antibody
- CRM1 homolog antibody
see all
图片
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ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded human breast tumor section by immunohistochemistry.
Detection: DAB staining -
ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded mouse squamous cell carcinoma by immunohistochemistry. Detection: DAB staining
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab84375 被引用在 1 文献中.
- Heerma van Voss MR et al. Nuclear DDX3 expression predicts poor outcome in colorectal and breast cancer. Onco Targets Ther 10:3501-3513 (2017). PubMed: 28761359