Application
Western blot
Sample
Human Cell lysate - whole cell (Human fetal retinal pigment epithelium)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Human fetal retinal pigment epithelium
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Licor Odyssey Clx
Negative control
HEK cells
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 35 kDa
Positive control
Human fetal retinal pigment epithelial cells (hfRPE)
Additional data
Additional Notes
Lane 1: Human fetal retinal pigment epithelium cells (hfRPE)
Lane 2: HEK cells
PVDF membrane
RIPA lysis buffer with mixed phosphatase and protease inhibitor cocktail
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提交于 Jul 10 2017